Table 3.
Substitutions | Temp / K | Yield b | λmax / nm c |
---|---|---|---|
none | 295 | 0.25 ± 0.02 | 343 |
Ala8ε-N-AcK d | 295 | 0.25 ± 0.01 | 341 |
Ala8Asn | 295 | 0.28 ± 0.03 | 344 |
Ala8Asp | 295 | 0.27 ± 0.01 | 344 |
Ala8Lys | 295 | 0.24 ± 0.01 | 343 |
Ala8Cha d | 295 | 0.27 ± 0.01 | 340 |
Ala8Gln | 295 | 0.23 ± 0.02 | 343 |
Ala8Glu | 295 | 0.25 ± 0.03 | 343 |
Ala8Ser | 295 | 0.22 ± 0.01 | 344 |
Ala8Cys | 295 | 0.036 ± 0.001 | 347 |
Ala8Orn d | 295 | 0.113 ± 0.005 | 345 |
Ala8Tyr | 295 | 0.134 ± 0.003 | 343 |
Ala8His | 295 | 0.17 ± 0.01 | 344 |
Phe10Tyr | 295 | 0.166 ± 0.008 | 344 |
Phe10His | 285 | 0.135 ± 0.004 | 347 |
Phe10Leu | 285 | 0.24 ± 0.03 | 345 |
Trp1Phe / Phe10Trp | 285 | 0.068 ± 0.005 | 345 |
The peptides are Ac-WVTIpGKAIFTG-NH2 with the indicated substitutions for residues in bold font. Except where indicated in the last row, all the peptides had Trp at position 1.
Fluorescence quantum yield (mean and standard deviation of three measurements).
Emission peak ± 1 nm.
Abbreviations: Ack = ε-N-Acetyl-lysine, Cha = cyclohexylalanine, Orn = ornithine.