FIGURE 10.

Long-term effects of Sertraline on caspase activity and viability of Min6 cells. A and B, Min6 cells remained untreated (open bars) or were treated with 30 mm LiCl (black bars) or BIO X (gray bars) for 1 h before being treated with 10 μm sertraline for 16 h. Caspase 3/7 activity (A) and cellular reducing power (B) were then determined as described under “Experimental Procedures.” C, Min6 cells were transfected with siRNA to JNK1/2 SMARTpools (50 nm) (black bars) or with non-targeting (NonT) control siRNAs (open bars). 48 h post-transfection, cells were treated with 10 μm sertraline for 16 h. Caspase 3/7 activity was then determined. D, Min6 cells were incubated for 16 h with the indicated concentrations of sertraline. Cells were collected, stained with propidium iodide (PI) or annexin V, and were analyzed by FACS. Outputs were gated and quantified for propidium iodide-positive cells (Q1), live cells (Q2), annexin V and PI-positive cells (Q3), or annexin V-positive cells (Q4). Data shown are mean ± S.E. of three (A and B) and two (C and D) experiments carried out in triplicates. *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared with controls.