FIGURE 2.

Transcriptional regulation of human MUC3 by the TFLK-containing 10-mer BP. A, shown is a schematic representation of the human MUC3 promoter constructs. The five deletion luciferase constructs that were generated to identify the sites of transcriptional regulation within the MUC3 promoter responded to the TFLK-containing 10-mer BP (10TFLK-BP). B, HT-29-Gal cells were transfected with the different luciferase constructs and treated or not treated with the TFLK-containing 10-mer BP. The cells were harvested after 48 h, and relative luciferase activity was determined using the Dual-Luciferase Reporter Assay System on a single sample luminometer. The data represent the mean ± S.E. of three independent experiments. * represents a significant difference (p < 0.05); ** represents a significant difference (p < 0.01).