FIGURE 2.

Effects of mutations in L1 (L22A) and/or L2 (L295A) on binding affinity of Mycd WT for CRM1. A, mixtures of HA-tagged CRM1, GTP-bound GST-FLAG-RanQ69L, and each indicated FLAG-tagged Mycd were subjected to IP analyses as described in the legend for Fig. 1. B, the respective IP/IB signal intensities were quantified as described under “Experimental Procedures.” The percentages indicate relative binding affinities for CRM1 normalized by the affinity of Mycd WT (wt), which was set at 100%. Results are means ± S.E. of three independent experiments (error bars).