FIGURE 5.

Effects of G-actin on interaction between CRM1 and MRTF-A. The indicated proteins were mixed with 10 μl (+) and 20 μl (++) of β-actin R62D, and their interactions were analyzed by the same procedures as described in the legend for Fig. 1 (upper panels). The respective IP/IB signal intensities were quantified as described under “Experimental Procedures.” The percentages indicate relative binding affinities for CRM1 normalized by the respective affinities of wild-type MRTF-A (A), MRTF-A L21A (the L1 mutant) (B), and MRTF-A L307A (the L2 mutant) (C) in the absence of β-actin R62D, which were set at 100% (lower panels). Results are means ± S.E. of three independent experiments (error bars).