FIGURE 3.

Medullary ammonium accumulation is altered in Rhcg^−/− mice. Kidneys from all three genotypes were dissected after 4 days of HCl load. Cortex (C), outer medulla (OM), and inner medulla (IM) were separated, and tissue ammonium content was measured. In wild type and heterozygous mice, a gradient in renal tissue ammonia content from cortex to inner medulla was observed. In Rhcg^−/− mice, the ammonia content increased from cortex to outer medulla, but ammonium content was lower in the inner medulla than in mice from the other two genotypes. (n = 5–8 mice/genotype), * statistically different from Rhcg^+/+ mice (p < 0.05). NS, not significant.