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Transepithelial NH3 permeability is reduced in the cortical collecting duct from Rhcg+/− and Rhcg−/− mice. Transepithelial permeability to NH3 was assessed in in vitro isolated and microperfused cortical collecting ducts from mice kept for 2 days on an HCl diet by imposing a bath-to-lumen NH3 gradient (see under “Experimental Procedures”) (*, p < 0.05).
