TABLE 1.
Theoretical and observed masses of the parent ion containing the phenylhydrazine-derivatized LTQ cross-link
Mass (theoretical) | Mass (observed) | Parent iona,b,c |
---|---|---|
1739.2 | 1738.7 | (α+β+PH+3H)3+ |
1304.7 | 1304.6 | (α+β+PH+4H)4+ |
1043.9 | 1044.2 | (α+β+PH+5H)5+ |
a α = peptides 670–690, containing Tyr-689 of the LTQ cofactor, minus 2H+.
b β = peptides 648–669, containing Lys-653 of the LTQ cofactor, minus H+.
c PH = phenyl–N=N–.