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MRK interacts with Rho proteins in vitro. KT3-MRK was immunoprecipitated with KT3 antibodies from M28 cells grown in serum-free medium and incubated in vitro with purified RhoC or RhoA proteins loaded with GDP or GTP-γS. ROCKII protein was used as a positive control for RhoC-binding. Rho proteins were detected using the X-Press antibody that recognizes their tag.
