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Down-regulation of MRK or RhoC increases focal adhesion number and length as well as cell area. ES-2 cells were transfected with the indicated siRNAs, 48 h later they were plated on coverslips and 24 h later processed for immunofluorescence. Histograms are means + S.E. of data from 15–30 cells and are representative of two independent experiments. L: luciferase; A: RhoA; C: RhoC; M: MRK. *, p < 0.02; **, p < 0.001.
