FIGURE 4.

Stoichiometry of the interaction between IT4VAR13^DBLβ and ICAM-1. Stoichiometry was determined by analytical SEC (a) and ultracentrifugation (b). mAu, milliabsorbance units. a, shown is a size-exclusion chromatogram of IT4VAR13^DBLβ (gray, wide dashes), ICAM-1^D1D5 (black, short dashes) and IT4VAR13^DBLβ·ICAM-1^D1D5 (black, continuous line). b, shown is continuous sedimentation coefficient distribution that best describes the AUC data for IT4VAR13^DBLβ incubated with ICAM-1^D1D5 for 30 min before centrifugation with the continuous sedimentation coefficient distribution for IT4VAR13^DBLβ inset for comparison. The distribution for ICAM-1^D1D5 is in Fig. 3c.