TABLE 3.
Summary of AUC sedimentation velocity results
Theoretical molecular weights of monomeric and 1:1 species are given in parentheses.
| Frictional coefficient (f/f0) | Sedimentation coefficient (S) | MWapp | Predicted species | |
|---|---|---|---|---|
| IT4VAR13 (314 kDa) | 1.66 | 4.2 | 89.1 | Truncation |
| 9.2 | 286.9 | IT4VAR13 monomer | ||
| 13.1 | 488.7 | IT4VAR13 dimer | ||
| ICAM-1D1D5 (50 kDa) | 1.67 | 3.2 | 63.4 | Monomer |
| 4.8 | 118.0 | Dimer | ||
| 6.8 | 197.0 | Tetramer | ||
| IT4VAR13:ICAM-1D1D5 (364 kDa) | 1.74 | 3.3 | 71.0 | ICAM-1D1D5 monomer |
| 9.6 | 333.1 | 1:1 IT4VAR13:ICAM-1D1D5 | ||
| 13.0 | 479.4 | IT4VAR13 dimer | ||
| IT4VAR13DBLβ (45 kDa) | 1.37 | 3.1 | 51.9 | Monomer |
| 4.8 | 114.0 | Dimer | ||
| IT4VAR13DBLβ:ICAM-1D1D5 (95 kDa) | 1.53 | 3.4 | 51.0 | Unbound monomeric IT4VAR13DBLβ/ ICAM-1D1D5 |
| 5.9 | 120.0 | 1:1 IT4VAR13DBLβ-ICAM-1D1D5 |