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. 2013 Jan 7;288(8):5840–5848. doi: 10.1074/jbc.M112.422501

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Concentration-dependent inhibition of PLCδ1 by 3013 at two different concentrations of PtdIns(4,5)P₂. Purified PLCδ1 was incubated with the indicated concentrations of 3013 in the presence of two different concentrations (16 and 80 μm) of the PtdIns(4,5)P₂ substrate. Phospholipase activity was calculated as described under “Experimental Procedures.”