FIGURE 2.

Binding of Arabidopsis XRCC1 to DNA. A–D, increasing concentrations of XRCC1 (0, 1, 2, 4, 6, and 8 μm) were incubated with a fluorescently labeled (*) homoduplex DNA (A) or with an analogous duplex containing a single 5-meC:G pair (B), a single-nucleotide gap flanked by 3′-phosphate and 5′-phosphate termini (both indicated by P) (C), or a single-nucleotide gap flanked by 3′-OH and 5′-deoxyribose-5-phosphate (dRP) termini (D). After nondenaturing gel electrophoresis, protein-DNA complexes were identified by their retarded mobility when compared with that of free DNA, as indicated.