FIGURE 3.

Mutation rates and spectrum of hPol δ^A692Q at the lacZ locus on M13mp2 gapped plasmid. A, mutations detected in the gapped plasmid filling assay are mapped on the scorable region of the gene. Base substitutions appear above the line, whereas −1 deletions (Δ) or +1 insertions (+N) are found below the line. B, mutation rates from 188 mutants sequenced, including individual base substitutions, ±1 frameshifts and overall base substitution rate, calculated as described under “Experimental Procedures.”