FIGURE 1.
Thermostability of 5-HT3R at different stages of purification measured by radioligand binding. Samples containing 5-HT3R were collected after extraction with C12E9, purification, and reconstitution into lipid bilayers, and after a 30-min thermal challenge, the remaining activity was determined by radioligand binding at 4 °C. A, thermostability during purification from cell homogenate, membrane fraction, and solubilized membranes to purified 5-HT3R. B, thermostability during reconstitution (Rec.) from purified 5-HT3R, ternary mixture, and proteoliposomes to cell homogenate. C, apparent Tm of indicated samples.