TABLE 3.

Kinetic parameters for the eIF1-eIF1A FRET change and GTP hydrolysis in 43 S PICs

eIF1A variant	eIF1 release kobs	GTP hydrolysis kobs
	s−1	s−1
WT	k1 = 4 ± 1	k1 = 14 ± 4
	a1 = 0.6 ± 0.05	a1 = 0.7 ± 0.1
	k2 = 0.6 ± 0.1	k2 = 0.4 ± 0.1
	a2 = 0.4 ± 0.05	a2 = 0.3 ± 0.1
	k1 = 3 ± 1	k1 = 1.4 ± 0.6
SE2*	a1 = 0.6 ± 0.1	a1 = 0.4 ± 0.1
	k2 = 0.7 ± 0.1	k2 = 0.03 ± 0.01
	a2 = 0.4 ± 0.1	a2 = 0.6 ± 0.1
	k1 = 1 ± 0.5	k1 = 0.7 ± 0.2
SE1*,SE2*	a1 = 0.5 ± 0.1	a1 = 0.2 ± 0.1
	k2 = 0.4 ± 0.1	k2 = 0.02 ± 0.005
	a2 = 0.5 ± 0.1	a2 = 0.8 ± 0.1