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. 2013 Jan 1;288(8):5426–5442. doi: 10.1074/jbc.M112.431569

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Analysis of metal concentrations in different E. coli strains. Total crude extracts and membrane fractions were prepared from E. coli strains BW25113, JW3435 (ΔtusA), JW1119 (ΔmnmA), and JW0764 (ΔmoaA). ICP-OES was used to analyze levels of molybdenum (black), iron (light gray bars), nickel (dark gray bars), and copper (gray bars) in total crude extract (A) and in the membrane fraction of each strain (B). The metal content of the wild type strain was set to 100%. All metal contents were related to the total protein concentration. Results were derived from at least three independent measurements, and standard deviations are within a range of 20%.