TABLE 5.
Analysis of the activity of hydrogenase, EcTMAOR, and EcNR in the E. coli ΔtusA strain
Activites were measured as units/mg. For E. coli hydrogenases, specific enzyme activity is defined as 1 μmol of benzyl viologen reduced min−1mg−1. For EcTMAOR and EcNR, specific enzyme activity is defined as the oxidation of 1 μmol of benzyl viologen min−1 mg−1.
Enzyme | BW25113 | ΔtusA | ΔmnmA |
---|---|---|---|
Units/mg | Units/mg | Units/mg | |
E. coli hydrogenases | 6.56 ± 0.46 | 25.99 ± 2.25 | 9.12 ± 1.76 |
E. coli TMAOR | 1.73 ± 0.04 | 0.07 ± 0.01 | 1.75 ± 0.05 |
E. coli NR | 2.13 ± 0.05 | 0.13 ± 0.01 | 2.17 ± 0.06 |