TABLE 7.
SPR measurement of protein-protein interactions using IscS, TusA, and IscU
| Immobilized proteina | RUb | Protein partnerc | KDd | chi2 |
|---|---|---|---|---|
| μm | ||||
| IscS | 1118 | BSA | NDe | |
| IscS | 1118 | TusA | 0.966 | 1.41 |
| IscS | 1118 | IscU | 14.6 | 0.363 |
| IscS | 1118 | IscS | 0.995 | 7.3 |
| IscU | 1049 | BSA | NDe | |
| IscU | 1049 | TusA | NDe | |
| IscU | 1049 | IscU | 2.49 | 0.142 |
| IscU | 1049 | IscS | 0.574 | 6.14 |
| TusA | 538 | BSA | NDe | |
| TusA | 538 | TusA | NDe | |
| TusA | 538 | IscU | NDe | |
| TusA | 538 | IscS | 0.304 | 0.949 |
a Proteins were immobilized via amine coupling.
b RU, resonance units.
c Proteins were injected using the KINJECT protocol, injecting samples in a concentration range of 0.4 to 20 μm. Cells were regenerated by injection of 20 mm HCl.
d KD values were obtained by global fitting procedures for a 1:1 binding.
e ND, no binding detectable (<1 RU).