The elution order of PCB 174 atropisomers on the BDM, BGB, CB and CD columns reveals an inversion in the elution of the atropisomers on the CD column compared to BDM, BGB and CB columns. (A) Incubation, BDM column, EF = 0.44; (B) racemic standard, BDM column, EF = 0.48; (C) incubation, BGB column, EF = 0.45; (D) racemic standard, BGB column, EF = 0.48 (E) incubation, CB column, EF = 0.45; (F) racemic standard, CB column, EF = 0.48; (G) incubation, CD column, EF = 0.55; (H) racemic standard, CD column, EF = 0.50.