Table 1. Primers used to amplify the specific insert.
| Sequence variant | Primer | Sequence 5′->3′ | Size (bp) |
| c.6_9dup (p.L4DfsX39)MYO7A | L4DfsX39_D_XhoI | AAGAAT CTCGAG AGTGGCTGAGAGAAGAATTC | 563 |
| L4DfsX39_R_NheI | AAGAAT GCTAGC GGATCCAGAGATGTGTGTAA | ||
| c.470G>A (p.S157N) MYO7A | S157N_D_XhoI | AAGAAT CTCGAG AGACTCCACCTCCCTCTTCA | 668 |
| S157N_R_NheI | AAGAAT GCTAGC GCTAATGTGAGCTTTGGTAGC | ||
| c.640G>A (p.G214R) MYO7Ac.721C>G (p.R241G) MYO7A | G214R & R241G_D_XhoI | AAGAAT CTCGAG GAAGGTGAAGGAGAGTGCG | 703 |
| G214R & R241G_R_NheI | AAGAAT GCTAGC TTCATGGTGGGATTTCCAGC | ||
| c.1097T>C (p.L366P)MYO7A | L366P_D_XhoI | AAGAAT CTCGAG TGACATGCTGGAGGGAGTTA | 608 |
| L366P_R_NheI | AAGAAT GCTAGC CTGACCAAAGCAGGCCAAAA | ||
| c.1342_1343delAG (p.S448LfsX2) MYO7A | S448LfsX2_D_XhoI | AAGAAT CTCGAG TCTCCAGGCAGAGGGAACAG | 524 |
| S448LfsX2_R_NheI | AAGAAT GCTAGC AGCCAGGCTCAGCTGACCTT | ||
| c.3508G>A (p.E1170K) MYO7A | E1170K_D_XhoI | AAGAAT CTCGAG GAGGTGCTTTATGCCCGATG | 545 |
| E1170K_R_NheI | AAGAAT GCTAGC GAGTGTGGGGAAAAGAGGTG | ||
| c.3652G>A (p.G1218R) MYO7A | G1218R_D_XhoI | AAGAAT CTCGAG ATAGATGGTGGAGCTGAGAG | 370 |
| G1218R_R_NheI | AAGAAT GCTAGC GCACTGGACACACACACACA | ||
| c.5581C>T (p.R1861X) MYO7A | R1861X_D_XhoI | AAGAAT CTCGAG ACTAGTTGCATCTGGCTGTC | 543 |
| R1861X_R_NheI | AAGAAT GCTAGC ATCCTGAGCAGCTGCAGGAT | ||
| c.1086-12G>A USH1C | USH1C_D_XhoI | AAGAAT CTCGAG ACAAGCTCGGGGACTTTGCT | 438 |
| USH1C_R_NheI | AAGAAT GCTAGC ATGGTCTCTGACCCACAGCT | ||
| c.2289+1G>A CDH23 | c.2289_D_XhoI | AAGAAT CTCGAG CAAATATTTCCTCCTCATGG | 636 |
| c.2289_R_NheI | AAGAAT GCTAGC GCAGGAGAGAAAGTATTTCA | ||
| c.6049G>A (p.G2017S) CDH23 | G2017_D_XhoI | AAGAAT CTCGAG CTTGCTCACCACTCTCATGT | 594 |
| G2017_R_NheI | AAGAAT GCTAGC GTATCGTCTCCAAGCCTCTT | ||
| c.8722+1delG CDH23 | c.8722_D_XhoI | AAGAAT CTCGAG TTCTGGGCAACACTCCGTGA | 1226 |
| c.8722_R_NheI | AAGAAT GCTAGC GGATGGACGGATAACTAATGG | ||
| c.1304_1305insC PCDH15 | c.1304_D_XhoI | AAGAAT CTCGAG CAGAAGACTGAAGCAATTAAGCC | 647 |
| c.1304_R_NheI | AAGAAT GCTAGC ATATACTGAATATCTGCAAGCTG | ||
| c.2868+5G>A PCDH15 | c.2868_D_XhoI | AAGAAT CTCGAG ATTCCAGTGGAACGGCCTTC | 533 |
| c.2868_R_NheI | AAGAAT GCTAGC TATGCTCTGTACCTGTGGATG | ||
| c.3717+2dupTT PCDH15 | c.3717_D_XhoI | AAGAAT CTCGAG CAGAATTATACCTATGTCCC | 635 |
| c.3717_R_NheI | AAGAAT GCTAGC AGTGACTAACAATCTGAGTG | ||
| c.521A>G (p.N174S) PCDH15 | N174S_D_XhoI | AAGAAT CTCGAG TTCTTCGCCCATAGCAAGA | 605 |
| N174S_R_NheI | AAGAAT GCTAGC TGAGGCATATTATACCTATG | ||
| c.1737C>G (p.Y579X) PCDH15 | Y579X_D_XhoI | AAGAAT CTCGAG AGTATTGTAACAGGACACAG | 596 |
| Y579X_R_NheI | AAGAAT GCTAGC CCTCTGATATTGTCCTCTTC |
Tails added at the beginning of the primer are indicated in bold.
Enzyme restriction sites used in this study are indicated in italics.