Table 3. Primers used to amplify fragments of MYO7A and CDH23 cDNAs from nasal cells.

Gene	Exons	Primers	Sequence 5′->3′	Size(bp)
MYO7A	1–5	MYO_EXP_1-5-EXT-D	AGAGACAAGAGACACACACA	628
		MYO_EXP_1-5-EXT-R	CTTCTTGTTGGTATACTGGC
		MYO_EXP_1-5-INT-D	TAGAACGAGACTTGGAGCCA	490
		MYO_EXP_1-5-INT-R	TTCACAGCCACCAGGATGGA
	3–9	MYO_EXP_3-9-EXT-D	ACCATGTGTGGATGGACCTG	937
		MYO_EXP_3-9-EXT-R	CTTCGAGATCTCCCAGTTCT
		MYO_EXP_3-9-INT-D	ACTCTGGGCAGGTCCAGGT	806
		MYO_EXP_3-9-INT-R	TGTTGGCGTACTCCTGGCT
	8–14	MYO_EXP_8-14-EXT-D	TGTTCTACTGCATGCTGGAG	906
		MYO_EXP_8-14-EXT-R	CCTGCAAAATGGTTGATGCC
		MYO_EXP_8-14-INT-D	AAGAAGAAGCTGGGTTGGG	811
		MYO_EXP_8-14-INT-R	TTGGCGTTGAGCTTGTGCT
	25–33	MYO_EXP_25-33-EXT-D	ATGAGACCCTGGGCAAGAAG	1136
		*MYO_EXP_25-33-EXT-R	CTTCTGGGCATCAGTTCTCC
		MYO_EXP_25-33-INT-D	AGGGCCAGAAGAAGAGCAGT	924
		MYO_EXP_25-33-INT-R	CGCTCCAGGATCATCTCAGA
	38–45	MYO_EXP_38-45-EXT-D	TCCTATGACTACTTCAGGCC	1010
		MYO_EXP_38-45-EXT-R	GGGGAAGTAGGACTTGTCCT
		MYO_EXP_38-45-INT-D	TCAAGCAGGCGCTGCTCAAGA	835
		MYO_EXP_38-45-INT-R	CGTGCACTTGTGGTAGCCTC
CDH23	18–24	CDH_EXP_18-24-EXT-D	ATGAACAGATATCCAATGGGC	790
		CDH_EXP_18-24-EXT-R	GGTTCTGAAAGGTGGGGTCA
		CDH_EXP_18-24-INT-D	AATGACAACCCTCCCACCTT	627
		CDH_EXP_18-24-INT-R	CACTGCTGGTGGCTTTCAGA
	43–49	CDH_EXP_43-49-EXT-D	CATCAACGACAACGACCCTGT	1084
		CDH_EXP_43-49-EXT-R	GGTTGAGGTCGTGGTCAATG
		CDH_EXP_43-49-INT-D	TCTGTGAAGGACAACCCGGA	708
		CDH_EXP_43-49-INT-R	GAATGGTGACAATGGCTGTG
	57–63	CDH_EXP_57-63-EXT-D	CTCATCTTGGTGGCCAGCGAC	1015
		CDH_EXP_57-63-EXT-R	CCGGCAGCCGGACAGAGAT
		CDH_EXP_57-63-INT-D	TTCATCGTCAAGGCCTCCAG	713
		CDH_EXP_57-63-INT-R	TAGCTGCTCCTTGTTCTCA

^*The primer MYO_EXP_25-33-EXT-R was designed by Vaché et al. [11].