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. 2013 Feb 25;8(2):e57268. doi: 10.1371/journal.pone.0057268

Figure 1. Calibration of voltage sensitive dye DIBAC4(3) in MIO-M1 cells.

Figure 1

A- Representative experiment showing the response of cells previously loaded with 2.5 µM DIBAC4(3) for 15 minutes, exposed to different extracellular concentrations of NaCl. Points represent changes in fluorescence intensity relativized to the stationary values, in the absence of gramicidin (Ft/F0 DIBAC4(3)). When a stable signal was registered, control solution was replaced by a solution containing 5 µM gramicidin. Afterwards, extracellular NaCl concentration was replaced (0 mM, 70 mM and 126 mM). B- Relation between relative changes in fluorescence and membrane potential calculated from Equation 3.