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. 2013 Feb 25;8(2):e57268. doi: 10.1371/journal.pone.0057268

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© 2013 Fernández et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Representative kinetics of cell volume changes measured in BCECF-loaded MIO-M1 cells in response to hypoosmotic shock (ΔOsM = 100 mOsM) generated either by varying (HYPO_NaCl) or keeping constant extracellular ion composition (HYPO_Mannitol). Insert: Percentage of cell volume recovery at 10 minutes (% RVD₁₀) in both conditions. Values are mean ± SEM for 42–55 cells from 15 experiments, *p<0.05, HYPO_Mannitol vs. HYPO_NaCl.