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. 2013 Feb 25;8(2):e57268. doi: 10.1371/journal.pone.0057268

Figure 4. Role of NPPB-sensitive Cl− channels on RVD in MIO-M1 cells.

Figure 4

Representative cell volume changes measured in BCECF-loaded MIO-M1 cells in response to a hypoosmotic shock (ΔOsM = 100 mOsM) generated either keeping constant (HYPOMannitol) (A) or varying ion composition (HYPONaCl) (B). In all the experiments 10−4 M NPPB or vehicle (DMSO) was added to ISONaCl or ISOMannitol 10 minutes before the hypoosmotic shock and maintained during the entire experiment. C- % RVD10 after the hyposmotic challenge in DMSO or NPPB treated cells. Values are mean ± SEM for 28–76 cells from 5–13 experiments, *p<0.05, Vehicle vs. NPPB.