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| Composition | ISONaCl | HYPONaCl | ISOMannitol | HYPOMannitol |
| NaCl (mM) | 126 | 76 | 70 | 70 |
| KCl (mM) | 5.5 | 5.5 | 5.5 | 5.5 |
| CaCl2 (mM) | 2.5 | 2.5 | 2.5 | 2.5 |
| MgCl2 (mM) | 1.25 | 1.25 | 1.25 | 1.25 |
| Hepes (mM) | 20 | 20 | 20 | 20 |
| Glucose (mM) | 10 | 10 | 10 | 10 |
| Mannitol (mM) | 0 | 0 | 100 | 0 |
Osmolarity (mOsM,  ±SEM) |
299±2 | 200±2 | 293±2 | 195±1 |
