Table 2. Values of parameters used in simulations.
| Parameter | NaCl | Mannitol | Units |
| Cell Parameters | |||
| Vcell | 1×10−8 | 0.945×10−8 | cm3 |
| Ac | 2×10−5 | 2×10−5 | cm2 |
| PNa | 1××10−7 | 1×10−7 | cm.s−1 |
| PK | 2×10−6 | 2×10−6 | cm.s−1 |
| PKmax | 6×10−6 | 6×10−6 | cm.s−1 |
| PCl | 5×10−7 | 5×10−7 | cm.s−1 |
| PClmax | 5×10−6 | 5×10−6 | cm.s−1 |
| Pf | 1.5×10−2 | 1.5×10−2 | cm.s−1 |
| [Na]i | 0.184×10−4 | 0.195×10−4 | mol.cm−3 |
| [K] i | 1.32×10−4 | 1.31×10−4 | mol.cm−3 |
| [Cl] i | 0.291×10−4 | 0.226×10−4 | mol.cm−3 |
| [X] i | 1.10×10−4 | 1.16×10−4 | mol.cm−3 |
| Vm | −6.31×10−2 | −6.71×10−2 | Volts |
| τ | 20 | 20 | s (post-shock) |
| Isosmotic Solution | |||
| [Na]o | 1.26×10−4 | 0.76×10−4 | mol.cm−3 |
| [K]o | 0.055×10−4 | 0.055×10−4 | mol.cm−3 |
| [Cl]o | 1.315×10−4 | 0.815×10−4 | mol.cm−3 |
| [X]o | 0.27×10−4 | 1.27×10−4 | mol.cm−3 |
| Hyposmotic Solution | |||
| [Na]o | 0.76×10−4 | 0.76×10−4 | mol.cm−3 |
| [K]o | 0.055×10−4 | 0.055×10−4 | mol.cm−3 |
| [Cl]o | 0.82×10−4 | 0.815×10−4 | mol.cm−3 |
| [X]o | 0.27×10−4 | 0.27×10−4 | mol.cm−3 |
Data were obtained either from our own measurements in MIO-M1 cells or from literature, as follows: i- Vcell and Ac in isotonic conditions were estimated from confocal images as previously reported [43]; ii- Pf was obtained from the measured cell volume changes during a hypoosmotic challenge using a modification of the Fick’s law [20]; iii- Vm corresponds to the value recorded using sharp electrode patch clamp technique by Limb et al [19]; iv- intracellular Na+, K+ and Cl− concentration are the typical values for most cell types; v- extracellular Na+, K+ and Cl− concentrations are the same as in experimental solutions; vi- ion permeabilities were chosen in order to obtain the Vm value of MIO-M1 cells; vii- the initial parameters presented for the Mannitol condition were obtained by simulating an extracellular solution change, from ISONaCl to ISOMannitol (see Materials and Methods section).