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View full-text article in PMC

. 2013 Feb 25;8(2):e57458. doi: 10.1371/journal.pone.0057458

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© 2013 Matic et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Male and female liver tissue sections were analysed for gross structural morphology, lipid content and ERα protein expression. Hematoxylin and eosin (H&E) staining indicated CT and KO animals had similar gross liver morphology. Lipid staining revealed that similar amounts of lipid droplets were present in CT and LERKO animals. Staining for ERα indicated a predominant hepatic nuclear localisation, which was decreased in LERKO mice of both sexes. Sections from three individual 6 month-old mice from each of the test groups were analysed. The figure shows representative sections.