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. 2013 Feb 25;8(2):e57458. doi: 10.1371/journal.pone.0057458

Figure 5. HFD-fed LERKO mice show normal hormone profiles and insulin and glucose responses.

Figure 5

Eight month-old LERKO and CT mice were fed with a high fat or standard chow diets for 5 months, and changes in body weight (A), glucose tolerance (B) and circulating hormones (C) were assessed. Body weight increased steadily in HFD animals across both sexes. However, the increase in body weights was comparable in the CT and LERKO mouse groups within the respective genders (A). Male chow diet CT/LERKO n = 4; HFD CT/LERKO n = 8. Female chow CT/LERKO n = 7; HFD CT n = 6, LERKO n = 8. (B) IPGTT assessment of standard chow-fed and HFD regimented groups revealed that the HFD groups exhibited pronounced impaired glucose tolerance. However, comparable responses during IPGTT were observed between the respective male or female CT and LERKO mouse groups. Male chow CT/LERKO n = 4; HFD CT n = 7, LERKO n = 8. Female chow CT/LERKO n = 7; HFD CT n = 5, LERKO n = 7. (C) Compared to standard chow-diet, HFD feeding lead to increased insulin and IGF-1 levels both in CT and LERKO mice. However, circulating hormone levels were similar in CT and LERKO mice maintained on HFD or standard chow diet. n = 4–8. Additionally, significant difference was not observed for IPGTT based on calculating the area under the curve (data not shown).All data are presented as mean ±SD.