Figure 2. Quantification of cGMP accumulation.

A. Concentration-cGMP response curve for BAY 60-7550 in the presence of BAY 41-2272 in immature rat hippocampal slices. The EC₅₀ value was 50±9 nM and the Hill Slope was 0.65±0.04. B. Concentration-cGMP response curve for BAY 41-2722 in the presence of BAY 60-7550. EC₅₀ and Hill slope values were 6±3 µM and 0.96±0.12. In A and B, data shown in red were collected from slices that were additionally pre-treated with the NOS inhibitor L-NNA. Fits are logistic; adjusted R² statistics of the fits were >0.98. C. Effect of BAY compounds and the NO donor DEA/NO on cGMP. Data shown in white, blue and red were collected in parallel with the data shown in Figure 1C, 3A and 4A. Experimental conditions are indicated by the colour-coded labels. In every experiment, basal cGMP levels and the effect of L-NNA or the NO receptor antagonist ODQ on the cGMP response were measured. Basal levels were pooled across experiments. Asterisks: p<0.05 compared to basal by ANOVA with Dunnett's post hoc test. n = 4–12.