Skip to main content
. 2013 Feb 25;8(2):e57283. doi: 10.1371/journal.pone.0057283

Figure 4. PICC forms homodimers.

Figure 4

β-galactosidase activity as a reporter for interaction in a membrane yeast two-hybrid (split-ubiquitin) assay. PICC shows self-interaction as indicated by increased β-galactosidase activity in yeast containing the constructs Cub-PICC and NubG-PICC. β-galactosidase activity in yeast transformed with combinations of Cub-PICL or Cub-PICC with either the empty vector NubG or the unrelated gene Alg5-NubG were used as negative controls. Combinations of Cub-PICL or Cub-PICC with Alg5 fused to wild-type Nub (Alg5-NubI) were used as positive controls. a.u., arbitrary units. Mean values and standard deviation from 3 samples are shown.