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. 2013 Feb 25;8(2):e57807. doi: 10.1371/journal.pone.0057807

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© 2013 Shekarabi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, C and E) Tissue sections prepared from mouse testis were used in ISH detections with anti-sense Wnk1, anti-sense Wnk1/Hsn2 and sense Wnk1/Hsn2 (negative control) riboprobes, respectively. (B, D and F) Corresponding sections were stained with cresyl violet. Arrows in A and C indicate the increased expression of Wnk1 in the wall of the seminiferous tubule spermatogonia cell layers, in comparision to Wnk1/Hsn2. Sc = spermatocyte; SfT = seminiferous tubule; Sg = spermatogonia; Sz = spermatozoa.