Table 1. Intra-assay and inter-assay variability for the calculated copy number and cycle threshold (CT) for the three internal controls (IC).
| Intra-assay (n = 10) | Inter-assay (n = 38) | ||||||||||
| Copy number [Mean ± SD (%CV)] | |||||||||||
| S | T | T/S | S | T | T/S | ||||||
| IC high | 8032 ± 512 (6.4) | 36.1 ± 2.5 (7.0) | 4.5 ± 0.4 (8.9) | 7490 ± 453 (6.0) | 36.5 ± 3.0 (8.2) | 4.9 ± 0.4 (9.6) | |||||
| IC med | 2939 ± 250 (8.5) | 10.5 ± 0.6 (5.8) | 3.6 ± 0.4 (11.4) | 3082 ± 275 (8.9) | 9.4 ± 1.4 (15.5) | 3.0 ± 0.5 (16.7) | |||||
| IC low | 1899 ± 62 (3.2) | 5.8 ± 0.2 (3.7) | 3.1 ± 0.2 (5.0) | 1903 ± 73 (3.8) | 5.6 ± 0.2 (4.3) | 3.0 ± 0.2 (6.4) | |||||
| CT [Mean ± SD (%CV)] | |||||||||||
| S | T | T/S | S | T | T/S | ||||||
| IC high | 23.8 ± 0.1 (0.4) | 14.1 ± 0.1 (0.8) | --- | 23.9 ± 0.1 (0.3) | 14.5 ± 0.3 (1.8) | --- | |||||
| IC med | 25.3 ± 0.1 (0.5) | 16.0 ± 0.1 (0.6) | --- | 25.2 ± 0.3 (1.1) | 16.9 ± 0.4 (2.1) | --- | |||||
| IC low | 26.0 ± 0.05 (0.2) | 16.9 ± 0.1 (0.4) | --- | 26.0 ± 0.1 (0.4) | 17.7 ± 0.3 (1.7) | --- | |||||
Abbreviations: SD = standard deviation, CV = coefficient of variation, S = single copy gene, T = telomere repeats, T/S = relative telomere length ratio, IC = internal control, CT = cycle threshold