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A, Western blot and B, subsequent densitometry to show CASP2 and CASP3 protein levels after knockdown with relevant siRNA. C, and D, Immunocytochemistry to demonstrate CASP2 and CASP3 knockdown in DRGN and satellite cells by relevant siRNA, respectively. E, Quantification of DRGN and F, DRG glia survival after siRNA-mediated knockdown of CASP2 and CASP3. Scale bars in C and D = 50 µm.
