Figure 1. Creation and screening of hSCARB2-Tg mice.

(A) The human SCARB2 gene construct was used to create transgenic mice. The human SCARB2 was cloned under the hEF1α promoter and BGH poly A tail to obtain a pEF-1α-hSCARB2 expression vector, which was used for embryo microinjection. (B) Genomic PCR of the tail DNA of hSCARB2-Tg mice using primer set 1 (Table S1) was set to screen for the presence of the hSCARB2 transgene. The size of PCR and RT-PCR products is 175 bp. (C) Quantitative RT-PCR analysis of RNA using primer set 2 (Table S1) was extracted from different tissues of 8-wk-old and 1-wk-old transgenic mice and 8-wk-old non-transgenic mice as control to quantify hSCARB2 expression was performed. β-Actin gene expression in each tissue was used as the internal control. Each normalized 2^Ct value was ratio to the value from the mean of 2^Ct obtained from the muscle tissues of non-transgenic mice. A schematic representation of the hSCARB2 gene expression and the statistical average from 7 mice per group was shown.