Skip to main content
. 2013 Feb 25;8(2):e57843. doi: 10.1371/journal.pone.0057843

Figure 3. GPR158 promoter activity in TBM-1 cells following GC treatment.

Figure 3

(A) Schematic illustration of GPR158 promoter (−1053/+25 bp, from transcription start site) indicating the location of three GREs. (B and C) TBM-1 cells were cotransfected with GPR158 promoter construct and β-galactosidase plasmid using Lipofectamine LTX reagent, followed by Dex (B) or TA (C) treatment for indicated time points. (D) TBM-1 cells were cotransfected with promoter-less pGL3 vector and β-galactosidase plasmid. Post-transfection, the cells were either left untreated or treated with vehicle control, or Dex or TA. Treatment was for 3 days for TBM-1 cells. (B, C, and D) The luciferase activity was normalized to that of the promoterless pGL3 basic vector. Data are expressed as mean ± SEM of three independent experiments ***P<.001; **P<.01; *P<.05; ns, P>.05.