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. 2013 Feb 25;8(2):e57843. doi: 10.1371/journal.pone.0057843

Figure 4. GPR158 regulates the proliferation of TBM-1 cells.

Figure 4

TBM-1 cells were transfected at 70–80% confluence using Lipofectamine LTX reagent with either GPR158 expression plasmids or vector alone (A) OR either GPR158 siRNA or control scrambled siRNA as indicated (B and C) and incubated in growth medium for 3 days in a 6-well culture dishes. (A and C) After 3 days of transfection, the cells were trypsinized and counted using trypan blue dye in a hemocytometer chamber for the cells transfected with indicated plasmids. (B) Total RNA was isolated for analyzing the levels of GPR158 mRNA by qRT-PCR. β-actin was used as a reference gene. (A, B and C) The data represent mean ± SEM of three independent experiments. ***P<.001; **P<.01; *P<.05; ns, P>.05.