Table 1. Oligonucleotide primers used in this study.

Gene/Fragment	Method	Forward sequence	Reverse sequence
GPR158 (FraA)	PCR	ggccgctagcatgggagccatggcttaccc	tatagggcccctgattggggc
GPR158 (FraB)	PCR	tatagggccccggggcctgggcc	ggccgagctcattgtgaactgcaac agcc
GPR158 (FraC)	PCR	gcatatgctagcgagctcatcatc tctgctatattc	gcaggctctagactacactttaaaactatc cca
GPR158 promoter (-1053/+25bp)	PCR	ggccggtaccctcccttctccctcttttc	ggccgctagcgtaaggggtaagccatgg
NLS-M1	SDM	gacattcgggacgagctgcaacaactctatgcccaactg	cagttgggcatagagttgttgcagctcgt cccgaatgtc
NLS-M2	SDM	ctggaaatatataaaagacagcagatgatcacaaacaacccccac	gtgggggttgtttgtgatcatctgctgtcttttatatatttccag
GPR158	RT-PCR	atattgctacagaagcatatgag	atatttccagttgggcatagag
Myocilin	RT-PCR	gccagtttttgagtatgacct	gtttgttcgagttccagattc
Aquaporin-1	RT-PCR	tgccatcggcctctctgta	cagggttaatcccacagcca
β-actin	RT-PCR	cattgccgacaggatgcaga	ctgatccacatctgctggaa
GAPDH	RT-PCR	aacctgccaagtacgatgacatc	gtagcccaggatgcccttga

The abbreviations are: GPR158, G protein-coupled receptor 158; Fra, fragment; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PCR, polymerase chain reaction; RT-PCR, reverse transcriptase–polymerase chain reaction; SDM, site-directed mutagenesis.