Table 1. Sequences of qPCR primers for housekeeping genes.
Target gene | Species | 5′-primer sequence -3′ a) , b) | Product size (bp) | PCR efficiency | Reference | |
Forward | Reverse | |||||
GAPDH | Cj | TCGGAGTCAACGGATTTGGTC | TTCCCGTTCTCAGCCTTGAC | 181 | 0.920 | DD279474 |
Hs | --------------------- | -------------------- | 181 | 0.921 | AF261085 | |
ACTB | Cj | GATGGTGGGCATGGGTCAGAA | AGCCACACGCAGCTCGTTGT | 163 | 0.901 | DD279463 |
Hs | --------------------- | ---------------A---- | 163 | 0.883 | NM_001101 | |
HPRT | Cj | ATCCAAAGATGGTCAAGGTCG | GTATTCATTATAGTCAAGGGCATA | 134 | 0.842 | DD289567 |
Hs | --------------------- | ------------------------ | 134 | 0.880 | M31642 | |
B2M | Cj | CTATTCAGCATGCTCCAAAGA | AAGACAAGTCTGAATGCTCCAC | 168 | 0.928 | AF084623 |
Hs | ----C----G-A--------- | ---------------------- | 168 | 0.950 | AB021288 | |
UBC | Cj | TCCCTTCTCGGCGGTTCTG | . TGCATTGTCAAGCGGCGAT | 158 | 0.922 | AB571242 |
Hs | -------------A----- | TC----------T-A---- | 160 | 0.936 | NM_021009 | |
rRNA | Cj | CGACCATAAACGATGCCGAC | GGTGGTGCCCTTCCGTCAAT | 145 | 0.918 | AB571241 |
Hs | -------------------- | -------------------- | 145 | 0.940 | M10098 | |
SDHA | Cj | TGGGAACAAGAGGGCATCTG | CCACCACGGCATCAAATTCATG | 86 | 0.934 | XM_002745154 |
Hs | -------------------- | -------T-------------- | 86 | 0.948 | BC001380 | |
TBP | Cj | CCATGACTCCCGGAATCCCTAT | ATAGGCTGTGGGGTCAGTCCA | 70 | 0.920 | EU796973 |
Hs | ---------------------- | --------------------- | 70 | 0.954 | M55654 |
Hyphen indicates a nucleotide identical to human sequences.
Dot indicates a shift nucleotide to marmoset sequences.