Skip to main content
. 2013 Feb 25;8(2):e56296. doi: 10.1371/journal.pone.0056296

Table 1. Sequences of qPCR primers for housekeeping genes.

Target gene Species 5′-primer sequence -3′ a) , b) Product size (bp) PCR efficiency Reference
Forward Reverse
GAPDH Cj TCGGAGTCAACGGATTTGGTC TTCCCGTTCTCAGCCTTGAC 181 0.920 DD279474
Hs --------------------- -------------------- 181 0.921 AF261085
ACTB Cj GATGGTGGGCATGGGTCAGAA AGCCACACGCAGCTCGTTGT 163 0.901 DD279463
Hs --------------------- ---------------A---- 163 0.883 NM_001101
HPRT Cj ATCCAAAGATGGTCAAGGTCG GTATTCATTATAGTCAAGGGCATA 134 0.842 DD289567
Hs --------------------- ------------------------ 134 0.880 M31642
B2M Cj CTATTCAGCATGCTCCAAAGA AAGACAAGTCTGAATGCTCCAC 168 0.928 AF084623
Hs ----C----G-A--------- ---------------------- 168 0.950 AB021288
UBC Cj TCCCTTCTCGGCGGTTCTG . TGCATTGTCAAGCGGCGAT 158 0.922 AB571242
Hs -------------A----- TC----------T-A---- 160 0.936 NM_021009
rRNA Cj CGACCATAAACGATGCCGAC GGTGGTGCCCTTCCGTCAAT 145 0.918 AB571241
Hs -------------------- -------------------- 145 0.940 M10098
SDHA Cj TGGGAACAAGAGGGCATCTG CCACCACGGCATCAAATTCATG 86 0.934 XM_002745154
Hs -------------------- -------T-------------- 86 0.948 BC001380
TBP Cj CCATGACTCCCGGAATCCCTAT ATAGGCTGTGGGGTCAGTCCA 70 0.920 EU796973
Hs ---------------------- --------------------- 70 0.954 M55654
a)

Hyphen indicates a nucleotide identical to human sequences.

b)

Dot indicates a shift nucleotide to marmoset sequences.