. 2013 Feb 25;8(2):e56296. doi: 10.1371/journal.pone.0056296

Table 2. Sequences of qPCR primers for CD markers and cytokines.

`Target gene`	`Species`	`5′-primer sequence -3′` ^a) `,` ^b)		`Product size (bp)`	`PCR efficiency`	`Reference`
		`Forward`	`Reverse`
`CD3ε`	`Cj`	`GGCTTGCTGCTGCTGGTTTAC`	`CCGGATGGGCTCATAGTCTG`	`150`	`0.865`	`DQ189218`
	`Hs`	`---------------------`	`--------------------`	`150`	`0.848`	`NM_000733`
`CD4`	`Cj`	`GGAAAACGGGAAAGTTGCATCA`	`GCCTTCTCCCGCTTAGAGAC`	`163`	`0.926`	`AF452616`
	`Hs`	`C------A--------------`	`--------------C-----`	`162`	`0.907`	`M35160`
`CD8α`	`Cj`	`TCTCCCAAACCAAGTCCAAGG`	`AGTTTCTCAGGGCCGAGCAG`	`144`	`0.940`	`DQ189217`
	`Hs`	`---------A----C------`	`. ---G---------------`	`143`	`0.912`	`NM_001768`
`CD20`	`Cj`	`GGGCTGTCCAGATTATGAATG`	`GAGTTTTTCTCCGTTGCTGC`	`166`	`0.942`	`DQ189220`
	`Hs`	`---------------------`	`--------------------`	`166`	`1.002`	`X07203`
`IL-1β`	`Cj`	`TGCACCTGTACGATCCCTGAAC`	`TTGCACAAAGGACATGGAGAACAC`	`145`	`0.806`	`AB539804`
	`Hs`	`---------------A------`	`---T--------------------`	`145`	`0.780`	`NM_000576`
`IL-2`	`Cj`	`CCCAAGAAGGCCAAAGAATTG`	`CTTAAGTGAAAGTTTTTGCTTTGAG`	`104`	`0.773`	`DQ826674`
	`Hs`	`-------------C----C--`	`-------------------------`	`103`	`0.797`	`BC070338`
`IL-4`	`Cj`	`CATTGTCACAGAGCAAAAGACTC`	`CTCAGTTGTGTTCTTGGAGGCA`	`79`	`0.910`	`XM_002744606`
	`Hs`	`. GCC----------G-------`	`----------------------`	`77`	`0.878`	`NM_000589`
`IL-5`	`Cj`	`AATCACCAACTGTGCACTGAAGAA`	`. TTTGGCGGTCAATGTGTTCCTT`	`130`	`0.871`	`DQ658152`
	`Hs`	`------------------------`	`TT------C--------A--T---`	`132`	`0.860`	`NM_000879`
`IL-6`	`Cj`	`GATTCAATGAGGAGACTTGCC`	`TGTTCTGGAGGTACTCTAGGTA`	`81`	`0.920`	`DQ658153`
	`Hs`	`---------------------`	`----------------------`	`81`	`0.990`	`NM_00600`
`IL-10`	`Cj`	`CTGCCTCACATGCTTCGAGA`	`TGGCAACCCAGGTAACCCTTA`	`134`	`0.970`	`DQ658154`
	`Hs`	`------A-------------`	`---------------------`	`134`	`0.920`	`M57627`
`IL-12β`	`Cj`	`. GGACGGCAAGGAGTATGAGTA`	`TTGAGCTTGTGAACGGCATC`	`111`	`0.935`	`AB539805`
	`Hs`	`G----AA---------------`	`--------------------`	`112`	`0.900`	`M65272`
`IL-13`	`Cj`	`TCCAGCTTGCTTGTCCGAG`	`CTGCAAATAATGATGCGTT-GATGT`	`127`	`0.916`	`AB571243`
	`Hs`	`----------A--------`	`. ---------------T--C--A--`	`127`	`0.964`	`NM_002188`
`IFN-γ`	`Cj`	`GGGTTCTCTTGGCTGTTACTG`	`TGTCTAAGAAAAGAGTTCCATTATC`	`116`	`0.838`	`FJ598593`
	`Hs`	`---------------------`	`. -C----------------------`	`115`	`0.856`	`NM_000619`
`TNF-α`	`Cj`	`AGCCTGTAGCCCATGTTGTAG`	`CTCTCAGCTCCACGCCATTG`	`102`	`0.887`	`DQ520835`
	`Hs`	`---------------------`	`--------------------`	`102`	`0.817`	`NM_000594`

^a)

Hyphen indicates a nucleotide identical to human sequences.

^b)

Dot indicates a shift nucleotide to marmoset sequences.