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. 2013 Feb 25;8(2):e57695. doi: 10.1371/journal.pone.0057695

Figure 6. mycHIF3α reduces the number of Clara cells.

Figure 6

The expression of the Clara cell marker, Ccsp, was strongly decreased in mycHIF3α transgenic lungs at gestational age E18.5 compared to controls (A and C versus B and D). (E) Alveolar epithelial cell markers are downregulated in Hif3α transgenic lungs at gestational age E18.5 as shown by quantitative PCR. Epas1 (0,4 + 0.1 versus control 0.87 + 0.1, n = 3 each, P = 0.012), Aqp5 (0.33+ 0.1 versus control 0.96 + 0.1, n = 3 each, P = 0.005), Abca3 (0.25 + 0.1 versus control 0.92 + 0.1 n = 3 each, P = 0.002), Scd1(0.35 + 0.1 versus control 0.92 + 0.1, n = 3 each, P = 0.001). (F) There is no significant change in the mRNA expression of Hif1α gene (0,8 + 0.1 versus control 0.7 + 0.1, n = 3 each, P>0.05). Quantification of the number of (G) type II pneumocytes (Sftpd over Ttf1, 0.36 + 0.1 versus control 0.9 + 0.1; n = 3, P = 0.01) and (H) Clara cells (Ccsp over Ttf1, 0.3 + 0.1 versus control 0.82 + 0.1; n = 5, P = 0.01) showed a significant reduction of in the Hif3α double transgenic animals. White bars represent control lung samples, black bars represent mycHIF3α double transgenic lung samples. Scale bars: 100 µm (A, B) and 50 µm (C,D).