Figure 3. Roquin^gt/gt gene trap mice develop extensive small intestine and liver inflammation.

(A) The location of the β-geo gene trap cassette and genotyping primers relative to the Roquin exons. (B) Roquin^gt/gt mice were screened using gene trap-specific primers. Mice homozygous for the gene trap had a single band of 800 bp; heterozygotes had two bands of 800 bp and 486 bp; wild type mice had a single 486 bp band. Animal ages 16–22 weeks. (C) The Roquin protein was undetectable in the spleen of homozygous (gt/gt) mice by western blotting; 1animal each. Animal ages 16–22 weeks. (D) Roquin gene expression in the intestine of homozygous (gt/gt) mice was reduced by ∼95%; 1animal each. Animal ages 16–22 weeks. Representative H&E stained tissue sections of the (E) liver, (F) duodenum, (G) jejunum, (H) ileum, and (I) cecum of a Roquin^gt/gt mouse exhibited chronic hepatitis and intestinal inflammation. Pathology scores of sections are described in the text. E–I, original magnifications, 200×.