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. 2013 Feb 25;8(2):e57083. doi: 10.1371/journal.pone.0057083

Figure 4. Full length Sup35 protein is not necessary for SGs assembly and its localization into SGs.

Figure 4

(A) The distribution of Rpg1-GFP fusion protein under control (30°C) and heat shock (46°C) conditions in either the wild-type strain (Sup35p; CRY1011 strain) or the strain expressing only the N-terminal truncated form of Sup35 protein (Sup35ΔN; CRY1007 strain). SGs (Rpg1-GFP) were formed even in the mutant strain suggesting that the N-terminal part of Sup35 protein is dispensable for the SGs formation. Scale bar 4 µm. (B) Pellet and supernatant fractions from the wild-type (CRY998 strain) and the Sup35 N-terminal truncated mutant strains (CRY1001 strain) were analyzed by SDS-PAGE and Western blotting using the anti-Sup35 antibody. Samples were prepared as described elsewhere [2]. The N-terminal truncated form of Sup35 protein was enriched in the pellet fraction of heat-shocked cells, as well as the wild-type form of Sup35 protein, suggesting that only Sup35 C-terminal part is sufficient to localize this protein to the heat-induced stress granules.