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. 2013 Feb 25;8(2):e57001. doi: 10.1371/journal.pone.0057001

Figure 5. TbMCM-BP is required for full repression of silent RNA Pol I promoters in BF T. brucei.

Figure 5

(A) Diagrams of genes and their locations: a triple reporter in a silent BES, procyclic genes under a RNA Pol I promoter, and a procyclic gene within a RNA Pol II transcription unit. (B) TbMCM-BP deficiency increased expression of silent genes transcribed from RNA Pol I promoter. After 24 and 48 hr of post-Cre-induction, mRNA levels of each genes described in (A) were measured and normalized to TbURA3 mRNA level. Fold changes relative to Cre-uninduced cells (0 hr) are plotted. Standard deviations are shown as error bars. (C) Depletion of TbMCM-BP protein upon Cre induction by immunoblot. VSG3 expression was used as a positive control for RT-qPCR and immunoblot.