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. 2013 Feb 25;8(2):e57160. doi: 10.1371/journal.pone.0057160

Figure 2. Tube formation activity in vitro by low-passage primary melanoma cultures.

Figure 2

(A) Vessel-like networks in three-dimensional culture of cell lines: C8161 (HAG) and HUVEC (positive controls) and C81-61 (PAG) and NHEM (negative controls). Original magnification as indicated in images; (B) Vessel-like networks in three-dimensional culture of primary low-passage melanoma cultures: Mel-010 and Mel-14 (positive) and Mel-04 and Mel-001 (negative); (C) intracellular VEGF-A expression by indicated cells. Shaded histograms denote isotype control antibody only and empty histograms denote VEGF-A staining; (D) percentage of VE-cadherin positive cells as determined by flow cytometry. The data represents one of three experiments with equal results.