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. 2013 Feb 25;8(2):e57160. doi: 10.1371/journal.pone.0057160

Figure 5. Effect of NA on proliferation, invasion and cell cycle profile of melanoma cells.

Figure 5

(A) Net proliferation of HAG cells was quantified with standardized XTT test. The number of cells was determined 72 h after seeding. The number of the vehicle-treated control cells was determined as 100%. Figure shows a representative experiment out of four performed; (B) Invasion capacity of HAG cells was quantified by 24 h-matrigel invasion assay, with correction to proliferation. The invasion rate of the vehicle-treated control cells was determined as 100%. Figure shows a representative experiment out of three performed; (C) DNA flow cytometry of HAG cells in response to NA compared with vehicle-treated control cells-representative experiment; (D) Quantitative evaluation of the S+G2M subpopulation and the apoptotic cell fraction, measured as a subdiploid peak. Statistically differences compared with control. * indicate P<0.05, ** indicate P<0.01 (2-tailed t-test).