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. 2013 Feb 25;8(2):e57056. doi: 10.1371/journal.pone.0057056

Figure 5. KLK4 induced uPA expression in SKOV-3 cells.

Figure 5

A. Western blot analysis shows expression of uPA, α5 integrin (ITN) and KLK4 (V5) of 3 KLK4 and 3 vector control clones with native SKOV-3 cells as a control with GAPDH as a loading control. B. Western blot analysis shows expression of KLK4 and uPA in KLK4-1 clone transfected with siRNA KLK4 exon 1 (psilK4Ex1), and both KLK4 exon 1 and 2 knockdown constructs (psilK4Ex1+2), p-silencing scramble (psil Ctl) and mock controls. GAPDH was used as a loading control. C. Western blotting shows expression of KLK4 and uPA in KLK4-1 cells cultured as 2D-monolayers (2D), 3D-collagen I (Col I), 3D-Matrigel (Matrigel), and 3D-suspension (Susp), with GAPDH as a loading control. D. Western blot shows expression of KLK4 and uPA in serous EOC cells of primary tumors (T) and ascites (A) from 6 patients. WCL of OVCA432 MCAs serves as a positive control and GAPDH as a loading control. E. Densitometry analysis of 3 Western blots indicative of that shown in D. **P<0.05 and ***P<0.001 indicate the significantly different levels of KLK4 and uPA in ascitic (A) and primary tumor cells (T).