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. 2012 Dec 17;34(3):713–722. doi: 10.1093/carcin/bgs383

Fig. 2.

Fig. 2.

Overexpression of miR-124 inhibits the migration and invasion of breast cancer cell lines in vitro. (A) Wound healing assay of MDA-MB-231 and BT-549 cells transfected with NC or mature miR-124 mimics. A scratch wound was made and images from five different fields were taken. Representative pictures of one field at the beginning (t = 0) and at the end of the recording (t = 24h) in each condition are shown. (B) Transwell migration assay of MDA-MB-231 cells transfected with NC or mature miR-124 mimics. Cell migration was analyzed 24h after seeding in Transwells. (C) Dynamic monitoring of cell migration using the xCELLigence system. MDA-MB-231 cells transfected with NC or mature miR-124 mimics were seeded to a CIM-Plate and subjected to a dynamic migration assay lasting for 36h. (D) Matrigel invasion assay of MDA-MB-231 cells transfected with NC and mature miR-124 mimics. Cell invasion was analyzed 24h after seeding in Transwells. (E) Dynamic monitoring of cell invasion using the xCELLigence system. MDA-MB-231 cells transfected with NC or mature miR-124 mimics were seeded to a CIM-Plate precoated with Matrigel and subjected to a dynamic invasion assay lasting for 36h. Error bars indicate ±SEM. The symbols ** and *** represent great significant difference (P < 0.01 and P < 0.001) by a two-tailed Student’s t-test.