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. 2012 Dec 17;34(3):713–722. doi: 10.1093/carcin/bgs383

Fig. 5.

Fig. 5.

Overexpression of Slug impairs miR-124-induced inhibition of migration and invasion in MDA-MB-231 cells. (A) Morphology of MDA-MB-231 cells co-transfected with NC or miR-124 mimics together with either pcDNA3.1 (-)-vector or pcDNA3.1 (-)-Slug for 48h. Overexpression of Slug partially rescued the morphological change caused by ectopic expression of miR-124 in MDA-MB-231 cells. (B) Immunoblotting of Slug and EMT-related markers in MDA-MB-231 cells with the co-transfection of NC or miR-124 mimics together with either pcDNA3.1 (-)-vector or pcDNA3.1 (-)-Slug. (C) and (D) Dynamic monitoring of cell migration and invasion using the xCELLigence system. MDA-MB-231 cells co-transfected with NC or miR-124 mimics together with either pcDNA3.1 (-)-vector or pcDNA3.1 (-)-Slug. Overexpression of Slug impaired the reduction of migration (C) and invasion ability (D) caused by ectopic expression of miR-124 in MDA-MB-231 cells. Error bars indicate ±SEM. The symbol * denotes statistical difference (P < 0.05), whereas ** and *** represent great significant difference (P < 0.01 and P < 0.001) by a two-tailed Student’s t-test.